Coding

Part:BBa_K3003028:Design

Designed by: Aysenur Deniz Cayirtepe   Group: iGEM19_Bilkent-UNAMBG   (2019-10-16)


Ag43 Autotransporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 247
    Illegal PstI site found at 805
    Illegal PstI site found at 1405
    Illegal PstI site found at 2029
    Illegal PstI site found at 2300
    Illegal PstI site found at 2353
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 247
    Illegal PstI site found at 805
    Illegal PstI site found at 1405
    Illegal PstI site found at 2029
    Illegal PstI site found at 2300
    Illegal PstI site found at 2353
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 247
    Illegal PstI site found at 805
    Illegal PstI site found at 1405
    Illegal PstI site found at 2029
    Illegal PstI site found at 2300
    Illegal PstI site found at 2353
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 247
    Illegal PstI site found at 805
    Illegal PstI site found at 1405
    Illegal PstI site found at 2029
    Illegal PstI site found at 2300
    Illegal PstI site found at 2353
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1807
    Illegal NgoMIV site found at 1828
    Illegal AgeI site found at 823
    Illegal AgeI site found at 1567
    Illegal AgeI site found at 1981
    Illegal AgeI site found at 2223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

E.coli genome

References

Ahan, R. E., Kırpat, B. M., Saltepe, B., & Şeker, U. Ö. Ş. (2019). A Self-Actuated Cellular Protein Delivery Machine. ACS Synthetic Biology, 8(4), 686–696. https://doi.org/10.1021/acssynbio.9b00062